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G3BP1 抗体 (pSer232)

This 兔 多克隆 antibody specifically detects G3BP1 in WB, IHC 和 IF. It exhibits reactivity toward 人.
产品编号 ABIN362030
发货至: 中国
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Quick Overview for G3BP1 抗体 (pSer232) (ABIN362030)

抗原

See all G3BP1 抗体
G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

适用

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宿主

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克隆类型

  • 74
  • 30
多克隆

标记

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This G3BP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

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    pSer232

    特异性

    The antibody detects endogenous level of G3BP-1 only when phosphorylated at serine 232.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    免疫原

    Peptide sequence around phosphorylation site of pSer232 (S-S-S (p) -P-A) derived from Human G3BP-1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • 抗原

    G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

    别名

    G3BP-1

    背景

    May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

    分子量

    60 kDa

    NCBI登录号

    NP_005745

    UniProt

    Q13283

    途径

    SARS-CoV-2 Protein Interactome
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