The antibody detects endogenous level of PKD/PKCm only when phosphorylated at serine 738.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pSer738 (E-K-S (p) -F-R) derived from Human PKD, PKCm. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
抗原
PKC mu (PRKD1)
(Protein Kinase D1 (PRKD1))
别名
PKD/PKCmu
背景
Converts transient diacylglycerol. (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.