Phosphothreonine 抗体
Quick Overview for Phosphothreonine 抗体 (ABIN361757)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.
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纯化方法
- Peptide Affinity Purified
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免疫原
- Phosphothreonine conjugated to KLH
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anti-Phosphothreonine antibody (FITC)
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphothreonine antibody (Biotin)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphothreonine antibody (Atto 488)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphothreonine antibody (Atto 594)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphothreonine antibody (Atto 390)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphothreonine antibody (HRP)WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP
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应用备注
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- WB (1:500)
- ICC/IF (1:60)
- ELISA (1:2000)
- IP (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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2 μg/ml of ABIN361757 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- -20°C
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- Phosphothreonine
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物质类
- Amino Acid
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背景
- Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
抗原
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