HSP90 抗体
Quick Overview for HSP90 抗体 (ABIN361712)
抗原
See all HSP90 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Detects 90 kDa. This antibody is reactive with both the constitutive and the inducible form of HSP90. It does not bind to the native form and does not recognize HSP90 from E.coli or yeast.
-
交叉反应
- 小鸡, Fusarium, 人, Insect, 小鼠, 植物, 兔, 大鼠
-
纯化方法
- Protein G Purified
-
免疫原
- Heat shock protein 90 from the water mold Achyla ambisexualis
-
亚型
- IgG2b
-
-
anti-Heat Shock Protein 90 (HSP90) antibody
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 unconjugated
anti-Heat Shock Protein 90 (HSP90) (acLys284), (acLys292) antibodyHSP90 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90 (HSP90) (Lys284), (Lys292) antibodyHSP90 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90 (HSP90) antibodyHSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90 (HSP90) antibody (HRP)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 HRP
anti-Heat Shock Protein 90 (HSP90) antibody (FITC)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 FITC
anti-Heat Shock Protein 90 (HSP90) antibody (Biotin)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 Biotin
anti-Heat Shock Protein 90 (HSP90) antibody (PE)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 PE
anti-Heat Shock Protein 90 (HSP90) antibody (PerCP)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 PerCP
anti-Heat Shock Protein 90 (HSP90) antibody (APC)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 APC
-
-
应用备注
-
- WB (1:1000)
- IHC (1:2000)
- optimal dilutions for assays should be determined by the user.
-
说明
-
1 μg/ml of ABIN361711 was sufficient for detection of HSP90 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- -20°C
-
-
- HSP90 (Heat Shock Protein 90 (HSP90))
-
别名
- HSP90
-
背景
- HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immuno-adsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
-
基因ID
- 4768
-
UniProt
- Q8LLI5
-
途径
- M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
抗原
-
