Lysine (lys) (acetylated) 抗体 (Biotin)
Quick Overview for Lysine (lys) (acetylated) 抗体 (Biotin) (ABIN361691)
抗原
宿主
克隆类型
标记
应用范围
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抗原表位
- acetylated
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特异性
- Detects proteins containing acetylated lysine residues. No reaction to non-acetylated proteins.
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纯化方法
- Protein A Purified
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免疫原
- Acetylated KLH Conjugated
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anti-Lysine (lys) (acetylated) antibody
lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Lysine (lys) (acetylated) antibody (FITC)lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal FITC
anti-Lysine (lys) (acetylated) antibody (PE)lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal PE
anti-Lysine (lys) (acetylated) antibody (APC)lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal APC
anti-Lysine (lys) (acetylated) antibody (PerCP)lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal PerCP
anti-Lysine (lys) (acetylated) antibody (Atto 488)lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal Atto 488
anti-Lysine (lys) (acetylated) antibody (Atto 594)lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal Atto 594
anti-Lysine (lys) (acetylated) antibody (Atto 390)lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal Atto 390
anti-Lysine (lys) (acetylated) antibody (HRP)lys ICC, IF, IP, ELISA, WB 宿主: 兔 Polyclonal HRP
anti-Lysine (lys) (acetylated) antibodylys ICC, IF, IP, ELISA, WB, IHC 宿主: 小鼠 Monoclonal 7F8 unconjugated
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应用备注
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- WB (1:250)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1/250 dilution of SPC-156 was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Lysine (lys)
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别名
- Lysine
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物质类
- Amino Acid
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背景
- Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
抗原
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