DNAJB1 抗体
(2 references)Quick Overview for DNAJB1 抗体 (ABIN361662)
抗原
See all DNAJB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects ~40 kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.
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交叉反应
- Saccharomyces cerevisiae
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纯化方法
- Protein G Purified
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免疫原
- Full length protein HSP40 (YDJ1)
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亚型
- IgG1 kappa
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anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (C-Term) antibody
DNAJB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 1-340) antibodyDNAJB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 2-340) antibodyDNAJB1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 1-340) antibodyDNAJB1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal 3B9-E6 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB1770 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 74-123) antibodyDNAJB1 适用: 人, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody (HRP)DNAJB1 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal 3B9-E6 HRP
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody (FITC)DNAJB1 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal 3B9-E6 FITC
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应用备注
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- WB (1:2000)
- optimal dilutions for assays should be determined by the user.
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说明
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0.5 μg/ml of ABIN361661 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- -20°C
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: "Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock." in: Journal of proteomics, Vol. 206, pp. 103416, (2020) (PubMed).
: "The Hsp70 co-chaperone Ydj1/HDJ2 regulates ribonucleotide reductase activity." in: PLoS genetics, Vol. 14, Issue 11, pp. e1007462, (2019) (PubMed).
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: "Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock." in: Journal of proteomics, Vol. 206, pp. 103416, (2020) (PubMed).
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- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
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别名
- HSP40
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背景
- Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
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基因ID
- 855661
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NCBI登录号
- NP_014335
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UniProt
- P25491
抗原
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