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Tyrosine Hydroxylase 抗体 (pSer40)

Name: Tyrosine Hydroxylase 抗体 (pSer40)
Brand: antibodies-online
SKU: ABIN361503
Availability: OutOfStock

This anti-Tyrosine Hydroxylase antibody is a 兔多克隆 antibody detecting Tyrosine Hydroxylase in WB, IHC, ICC 和 ELISA. Suitable for 大鼠. This Primary Antibody has been cited in 12+ publications.

Western blots of recombinant phospho- and dephospho-TH showing selective immunolabeling by the phospho-specific antibody of the ~60k TH phosphorylated at Ser40. (Tyrosine Hydroxylase 抗体 (pSer40))

Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. (Tyrosine Hydroxylase 抗体 (pSer40))

IHC

Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. (Tyrosine Hydroxylase 抗体 (pSer40))

Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40. (Tyrosine Hydroxylase 抗体 (pSer40))

ISt

4 images

(12 references)

产品编号 ABIN361503

发货至: 中国

Quick Overview for Tyrosine Hydroxylase 抗体 (pSer40) (ABIN361503)

抗原

See all Tyrosine Hydroxylase (TH) 抗体

Tyrosine Hydroxylase (TH)

适用

大鼠

宿主

兔

克隆类型

多克隆

标记

This Tyrosine Hydroxylase antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA

Discover our top product TH Primary Antibody

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pSer40

特异性

Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over non-phospho TH.

交叉反应

哺乳动物, Various Species

产品特性

Our Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Tyrosine Hydroxylase (Ser40) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC.

纯化方法

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

免疫原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH).

亚型

IgG
anti-Tyrosine Hydroxylase (TH) (AA 101-165) antibody
TH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 82-310) antibody
TH 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer40) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer31) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) antibody
TH 适用: 人 WB, IF, ICC, IHC (p), IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 486-514) antibody
TH 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 34-301) antibody
TH 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer19) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 193-222), (Middle Region) antibody
TH 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (C-Term) antibody
Verified TH 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

应用备注

Dilution Range: ICC: 1:50-1:1000
Dilution Range: WB Brain: 1:1000
Dilution Range: WB: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

储存条件

-20 °C

储存方法

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

有效期

12 months
Salvatore, Calipari, Jones: "Regulation of Tyrosine Hydroxylase Expression and Phosphorylation in Dopamine Transporter-Deficient Mice." in: ACS chemical neuroscience, Vol. 7, Issue 7, pp. 941-51, (2017) (PubMed).

Salvatore, Terrebonne, Fields, Nodurft, Runfalo, Latimer, Ingram: "Initiation of calorie restriction in middle-aged male rats attenuates aging-related motoric decline and bradykinesia without increased striatal dopamine." in: Neurobiology of aging, Vol. 37, pp. 192-207, (2016) (PubMed).

Calipari, Ferris, Melchior, Bermejo, Salahpour, Roberts, Jones: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." in: Addiction biology, Vol. 19, Issue 2, pp. 145-55, (2014) (PubMed).

Izumi, Ezumi, Takada-Takatori, Akaike, Kume: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, (2014) (PubMed).

Salvatore, Pruett: "Dichotomy of Tyrosine Hydroxylase and Dopamine Regulation between Somatodendritic and Terminal Field Areas of Nigrostriatal and Mesoaccumbens Pathways." in: PLoS ONE, Vol. 7, Issue 1, pp. e29867, (2012) (PubMed).

McCutcheon, Conrad, Carr, Ford, McGehee, Marinelli: "Dopamine neurons in the ventral tegmental area fire faster in adolescent rats than in adults." in: Journal of neurophysiology, Vol. 108, Issue 6, pp. 1620-30, (2012) (PubMed).

Salvatore, Pruett, Dempsey, Fields: "Comprehensive profiling of dopamine regulation in substantia nigra and ventral tegmental area." in: Journal of visualized experiments : JoVE, Issue 66, (2012) (PubMed).

Perez-Costas, Melendez-Ferro, Rice, Conley, Roberts: "Dopamine pathology in schizophrenia: analysis of total and phosphorylated tyrosine hydroxylase in the substantia nigra." in: Frontiers in psychiatry, Vol. 3, pp. 31, (2012) (PubMed).

Nakashima, Mori, Kaneko, Hayashi, Nagatsu, Ota: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." in: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, (2011) (PubMed).

Meyer, Richer, Benkovic, Hayashi, Kansy, Hale, Moy, Kim, OCallaghan, Tsai, Greengard, Nairn, Cowan, Miller, Antich, Bibb: "Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18561-6, (2008) (PubMed).

Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).
抗原

Tyrosine Hydroxylase (TH)

别名

Tyrosine Hydroxylase

背景

Synonyms: Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody
Description Logic: Our Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific primary antibody is produced . It detects Tyrosine Hydroxylase (Ser40) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC.
Gene Name Alternatives: TH

分子量

60 kDa

基因ID

25085

UniProt

P04177

途径

Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour