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Tyrosine Hydroxylase 抗体 (pSer31)

Name: Tyrosine Hydroxylase 抗体 (pSer31)
Brand: antibodies-online
SKU: ABIN361502
Availability: OutOfStock

This anti-Tyrosine Hydroxylase antibody is a 兔多克隆 antibody detecting Tyrosine Hydroxylase in WB, IHC 和 IF. Suitable for 大鼠. This Primary Antibody has been cited in 5+ publications.

Western blots of PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60k TH phosphorylated at Ser31. (Tyrosine Hydroxylase 抗体 (pSer31))

Western blot of PC-12 cell lysate incubated in the absence (-) and presence (+) of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase phosphorylated at Ser31. (Tyrosine Hydroxylase 抗体 (pSer31))

2 images

(5 references)

产品编号 ABIN361502

发货至: 中国

Quick Overview for Tyrosine Hydroxylase 抗体 (pSer31) (ABIN361502)

抗原

See all Tyrosine Hydroxylase (TH) 抗体

Tyrosine Hydroxylase (TH)

适用

大鼠

宿主

兔

克隆类型

多克隆

标记

This Tyrosine Hydroxylase antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Discover our top product TH Primary Antibody

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pSer31

特异性

Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser31.

交叉反应

小鼠, 大鼠

预测反应

non-human primate

纯化方法

Antigen Affinity Purified from Pooled Serum

免疫原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 conjugated to KLH
anti-Tyrosine Hydroxylase (TH) (AA 101-165) antibody
TH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 82-310) antibody
TH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer40) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer31) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) antibody
TH 适用: 人 WB, IF, ICC, IHC (p), IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 486-514) antibody
TH 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 34-301) antibody
TH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (pSer19) antibody
TH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (AA 193-222), (Middle Region) antibody
TH 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tyrosine Hydroxylase (TH) (C-Term) antibody
Verified TH 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

应用备注

Recommended Dilution: WB: 1:1000 IF (frozen sections, Witkovsky et al., 2000): 1:1000 IHC (frozen sections, Witkovsky et al., 2000): 1:1000 Quality Control: Western blots performed on each lot.

限制

仅限研究用
状态

Liquid

缓冲液

100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

储存条件

-20 °C
Calipari, Ferris, Melchior, Bermejo, Salahpour, Roberts, Jones: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." in: Addiction biology, Vol. 19, Issue 2, pp. 145-55, (2014) (PubMed).

Izumi, Ezumi, Takada-Takatori, Akaike, Kume: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, (2014) (PubMed).

Nakashima, Mori, Kaneko, Hayashi, Nagatsu, Ota: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." in: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, (2011) (PubMed).

Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).
抗原

Tyrosine Hydroxylase (TH)

别名

Tyrosine Hydroxylase

背景

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982, Haycock et al., 1992, Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs. Anti-Phospho Ser31 Tyrosine Hydroxylase Western blot of PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60k TH phosphorylated at Ser31.

分子量

'60 kDa

基因ID

25085

UniProt

P04177

途径

Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour