Tryptophan Hydroxylase 1 抗体 (pSer58)
Quick Overview for Tryptophan Hydroxylase 1 抗体 (pSer58) (ABIN361500)
抗原
See all Tryptophan Hydroxylase 1 (TPH1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer58
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特异性
- Specific for the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.
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交叉反应
- 兔
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预测反应
- bovine, chicken, human, mouse, non-human primate, rat, xenopus, zebra fish
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纯化方法
- Antigen Affinity Purified from Pooled Serum
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免疫原
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
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anti-Tryptophan Hydroxylase 1 (TPH1) (Internal Region) antibody
TPH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 35-62), (N-Term) antibodyTPH1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB21510 unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 257-405) antibodyTPH1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (pSer58) antibodyTPH1 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 345-444) antibodyTPH1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) antibodyTPH1 适用: 兔 WB, IHC 宿主: 绵羊 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (N-Term) antibodyTPH1 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 26-75) antibodyTPH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 231-280) antibodyTPH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) antibodyTPH1 适用: 人 WB, IHC, IF/ICC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
- Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
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储存条件
- -20 °C
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- Tryptophan Hydroxylase 1 (TPH1)
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别名
- TPH1
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背景
- Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997). Anti-Phospho Ser58 Tryptophan Hydroxylase Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.
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分子量
- '55 kDa
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基因ID
- 24848
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UniProt
- P09810
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途径
- Feeding Behaviour
抗原
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