Synaptotagmin 抗体 (pSer309)
Quick Overview for Synaptotagmin 抗体 (pSer309) (ABIN361497)
抗原
See all Synaptotagmin (SYT) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer309
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特异性
- Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Ser 309. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
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交叉反应
- 大鼠
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预测反应
- bovine, canine, chicken, human, mouse, non-human primate, zebra fish
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产品特性
- Our Synaptotagmin (Ser309) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Synaptotagmin (Ser309) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.
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纯化方法
- Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
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免疫原
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).
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亚型
- IgG
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应用备注
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Dilution Range: ICC: 1:400
Dilution Range: WB Brain: 1:1000
Dilution Range: WB: 1:1000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
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储存条件
- -20 °C
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储存方法
- Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
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有效期
- 12 months
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: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).
: "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo." in: Nature, Vol. 426, Issue 6966, pp. 559-63, (2003) (PubMed).
: "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion." in: Nature, Vol. 424, Issue 6951, pp. 943-7, (2003) (PubMed).
: "Synaptotagmin I functions as a calcium regulator of release probability." in: Nature, Vol. 410, Issue 6824, pp. 41-9, (2001) (PubMed).
: "Regulation of synaptotagmin I phosphorylation by multiple protein kinases." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 921-32, (1999) (PubMed).
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: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).
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- Synaptotagmin (SYT)
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别名
- Synaptotagmin
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背景
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Synonyms: DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody
Description Logic: Our Synaptotagmin (Ser309) rabbit polyclonal phosphospecific primary antibody is produced . It detects Synaptotagmin (Ser309) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.
Gene Name Alternatives: SYT1
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分子量
- 60 kDa/62 kDa
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基因ID
- 25716
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UniProt
- P21707
抗原
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