RPH3A 抗体 (pSer234)
Quick Overview for RPH3A 抗体 (pSer234) (ABIN361489)
抗原
See all RPH3A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer234
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特异性
- Specific for the ~82k rabphilin protein phosphorylated at Ser234. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling
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交叉反应
- 大鼠
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预测反应
- mouse
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纯化方法
- Antigen Affinity Purified from Pooled Serum
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免疫原
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH
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应用备注
- Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
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储存条件
- -20 °C
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Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." in: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).
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Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." in: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).
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- RPH3A (Rabphilin 3A (RPH3A))
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别名
- RPH3A
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背景
- Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994,Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001). Anti-Phospho-Ser234 Rabphilin 3A Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.
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分子量
- '82 kDa
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基因ID
- 171039
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UniProt
- P47709
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途径
- Synaptic Vesicle Exocytosis
抗原
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