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RPH3A 抗体 (pSer234)

This anti-RPH3A antibody is a 兔 多克隆 antibody detecting RPH3A in WB 和 IHC. Suitable for 大鼠. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN361489
发货至: 中国

Quick Overview for RPH3A 抗体 (pSer234) (ABIN361489)

抗原

See all RPH3A 抗体
RPH3A (Rabphilin 3A (RPH3A))

适用

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大鼠

宿主

  • 43

克隆类型

  • 43
多克隆

标记

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This RPH3A antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

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    pSer234

    特异性

    Specific for the ~82k rabphilin protein phosphorylated at Ser234. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling

    交叉反应

    大鼠

    预测反应

    mouse

    纯化方法

    Antigen Affinity Purified from Pooled Serum

    免疫原

    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH
  • 应用备注

    Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

    储存条件

    -20 °C
  • Stephens, Powell, Taraska, Harris: "Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." in: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).

  • 抗原

    RPH3A (Rabphilin 3A (RPH3A))

    别名

    RPH3A

    背景

    Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994,Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001). Anti-Phospho-Ser234 Rabphilin 3A Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.

    分子量

    '82 kDa

    基因ID

    171039

    UniProt

    P47709

    途径

    Synaptic Vesicle Exocytosis
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