YWHAB 抗体 (pSer58)
Quick Overview for YWHAB 抗体 (pSer58) (ABIN361482)
抗原
See all YWHAB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer58
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特异性
- Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
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交叉反应
- 人, 大鼠
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预测反应
- bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus, zebra fish
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纯化方法
- Antigen Affinity Purified from Pooled Serum
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免疫原
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
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anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibody
YWHAB 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 5B5G10 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (Center) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibodyYWHAB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-141) antibodyYWHAB 适用: 人, 斑马鱼 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 34-221) antibodyYWHAB 适用: 人, 斑马鱼 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 56-85), (N-Term) antibodyYWHAB 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4062 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 217-246), (C-Term) antibodyYWHAB 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4064 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 51-150) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (C-Term) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Recommended Dilution: WB: 1:1000 IHC: 1:500 Quality Control: Western blots performed on each lot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
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储存条件
- -20 °C
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- YWHAB (14-3-3 alpha + beta (YWHAB))
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别名
- YWHAB
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背景
- 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004, Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003). Anti-Phospho-Ser58 14-3-3 Protein Western blot of rat brainstem lysate showing specific immuno- labeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).
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分子量
- '29 kDa
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基因ID
- 56011
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UniProt
- P35213
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location
抗原
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