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MARCKS 抗体 (pSer152, pSer156)

MARCKS 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN361478
发货至: 中国
  • 抗原 See all MARCKS 抗体
    MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
    抗原表位
    • 28
    • 20
    • 19
    • 12
    • 10
    • 8
    • 7
    • 5
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    • 4
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    • 1
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    • 1
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    pSer152, pSer156
    适用
    • 106
    • 75
    • 73
    • 10
    • 9
    • 8
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    大鼠
    宿主
    • 119
    • 3
    • 2
    克隆类型
    • 116
    • 8
    多克隆
    标记
    • 61
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This MARCKS antibody is un-conjugated
    应用范围
    • 72
    • 36
    • 27
    • 27
    • 21
    • 13
    • 13
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    特异性
    Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by -phosphatase
    交叉反应
    小鼠, 大鼠
    预测反应
    bovine,chicken, human, Xenopus, zebra fish
    纯化方法
    Antigen Affinity Purified from Pooled Serum
    免疫原
    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH
    Top Product
    Discover our top product MARCKS Primary Antibody
  • 应用备注
    Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
    储存条件
    -20 °C
  • 抗原
    MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
    别名
    MARCKS (MARCKS 产品)
    别名
    80K-L antibody, MACS antibody, PKCSL antibody, PRKCSL antibody, KINC antibody, Macs antibody, marcks antibody, MGC89127 antibody, MARCKS antibody, macs antibody, wu:fb55c06 antibody, wu:fb92d04 antibody, wu:fc19b04 antibody, wu:fc44a10 antibody, zgc:110235 antibody, fc34g03 antibody, fc38c06 antibody, wu:fc34g03 antibody, wu:fc38c06 antibody, zgc:109978 antibody, myristoylated alanine rich protein kinase C substrate antibody, myristoylated alanine-rich protein kinase C substrate antibody, methyl binding domain106 antibody, myristoylated alanine-rich protein kinase C substrate b antibody, myristoylated alanine-rich protein kinase C substrate S homeolog antibody, myristoylated alanine-rich protein kinase C substrate a antibody, MARCKS antibody, Marcks antibody, marcks antibody, mbd106 antibody, marcksb antibody, marcks.S antibody, marcksa antibody
    背景
    Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994). Anti-Phospho Ser152/156 MARCKS Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Serr152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.
    分子量
    '87 kDa
    基因ID
    3028
    UniProt
    P30009
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