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AQP2 抗体 (pSer261)

There are 2+ publications for this product available. The 兔 多克隆 anti-AQP2 antibody is suitable to detect AQP2 in samples from 大鼠. It has been validated for WB, IHC, ELISA, IP 和 ICC.
产品编号 ABIN361469
发货至: 中国
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Quick Overview for AQP2 抗体 (pSer261) (ABIN361469)

抗原

See all AQP2 抗体
AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This AQP2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
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    pSer261

    特异性

    Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

    交叉反应

    人, 小鼠, 大鼠

    预测反应

    bovine, canine, chicken, human, non-human primate

    产品特性

    Our Aquaporin 2 (Ser261) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Aquaporin 2 (Ser261) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC, IP.

    纯化方法

    Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

    免疫原

    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 of rat aquarporin 2, conjugated to keyhole limpet hemocyanin (KLH).

    亚型

    IgG
  • 应用备注

    Dilution Range: IP: 3 µL

    Dilution Range: ICC: 1:500-1:3000

    Dilution Range: WB: 1:1000

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

    储存条件

    -20 °C

    储存方法

    Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

    有效期

    12 months
  • Hoffert, Fenton, Moeller, Simons, Tchapyjnikov, McDill, Yu, Pisitkun, Chen, Knepper: "Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24617-27, (2008) (PubMed).

    Hoffert, Nielsen, Yu, Pisitkun, Schleicher, Nielsen, Knepper: "Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct." in: American journal of physiology. Renal physiology, Vol. 292, Issue 2, pp. F691-700, (2007) (PubMed).

  • 抗原

    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

    别名

    AQP2

    背景

    Synonyms: ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody

    Description Logic: Our Aquaporin 2 (Ser261) rabbit polyclonal phosphospecific primary antibody is produced . It detects Aquaporin 2 (Ser261) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC, IP.

    Gene Name Alternatives: AQP2

    分子量

    29 kDa/37 kDa

    基因ID

    25386

    UniProt

    P34080

    途径

    Response to Water Deprivation
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