Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by (-phosphatase treatment.
Recommended Dilution: WB: 1:1000 IF (fixed cultured neurons): 1:1000 IHC (fixed cultured neurons): 1:1000 Quality Control: Western blots performed on each lot.
限制
仅限研究用
状态
Liquid
缓冲液
100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001, Graham et al., 2002, Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.