Nuclei 抗体
(4 references)Quick Overview for Nuclei 抗体 (ABIN361360)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4 % Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.
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交叉反应
- 人
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产品特性
- Our Anti-Nuclei Antibody primary antibody is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in ELISA, IHC, ICC.
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纯化方法
- Concentrated tissue ulture supernatant.
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免疫原
- Human cell homogenate.
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亚型
- IgG1
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anti-Nuclei antibody
适用: 人 IHC, FM 宿主: 小鼠 Monoclonal 235-1 unconjugated
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应用备注
- 1:100
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说明
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Normally ABIN361360 should be fix with 10% buffered formalin overnight at room temperature instead of 4% paraformaldehyde. 1.Use 10% buffered formalin overnight instead of 4% paraformaldehyde to fix the tissues 2.Quench the endogenous peroxidase activity with 3% H2O2 instead of 0.5% H2O2 3.Block non-specific binding sites with 3% horse serum instead of goat serum.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Concentrated tissue culture supernatent
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
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有效期
- 12 months
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: "Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation." in: Nature communications, Vol. 10, Issue 1, pp. 5462, (2019) (PubMed).
: "Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo." in: Stem cell reports, Vol. 10, Issue 3, pp. 751-765, (2018) (PubMed).
: "Multimodal Approaches for Regenerative Stroke Therapies: Combination of Granulocyte Colony-Stimulating Factor with Bone Marrow Mesenchymal Stem Cells is Not Superior to G-CSF Alone." in: Frontiers in aging neuroscience, Vol. 6, pp. 130, (2014) (PubMed).
: "Rb suppresses collective invasion, circulation and metastasis of breast cancer cells in CD44-dependent manner." in: PLoS ONE, Vol. 8, Issue 12, pp. e80590, (2014) (PubMed).
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: "Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation." in: Nature communications, Vol. 10, Issue 1, pp. 5462, (2019) (PubMed).
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- Nuclei
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背景
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Synonyms: HNA antibody, Human Nuclear Antigen antibody, HuNu antibody
Description Logic: Our Anti-Nuclei Antibody primary antibody is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in ELISA, IHC, ICC.
Gene Name Alternatives: Marker for human cells in xenographic research
抗原
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