Nuclei 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in IF 和 IHC. Suitable for 人. This Primary Antibody has been cited in 4+ publications.
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(4 references)Quick Overview for Nuclei 抗体 (ABIN361360)
抗原
Nuclei
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC)
克隆位点
235-1
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特异性
- The antibody has been directly tested for reactivity in human. This antibody does not react with mouse or rat nuclei. Specific for human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.
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交叉反应
- 人
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纯化方法
- Concentrated tissue culture supernatant
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免疫原
- Human cell homogenate.
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亚型
- IgG1
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anti-Nuclei antibody
适用: 人 IHC, FM 宿主: 小鼠 Monoclonal 235-1 unconjugated
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应用备注
- Recommended Dilution: IF: 1:100 IHC: 1: 100. Quality Control: Immunofluorescence performed on each lot.
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说明
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Normally ABIN361360 should be fix with 10% buffered formalin overnight at room temperature instead of 4% paraformaldehyde. 1.Use 10% buffered formalin overnight instead of 4% paraformaldehyde to fix the tissues 2.Quench the endogenous peroxidase activity with 3% H2O2 instead of 0.5% H2O2 3.Block non-specific binding sites with 3% horse serum instead of goat serum.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- total IgG fraction
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储存条件
- -20 °C
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: "Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation." in: Nature communications, Vol. 10, Issue 1, pp. 5462, (2019) (PubMed).
: "Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo." in: Stem cell reports, Vol. 10, Issue 3, pp. 751-765, (2018) (PubMed).
: "Multimodal Approaches for Regenerative Stroke Therapies: Combination of Granulocyte Colony-Stimulating Factor with Bone Marrow Mesenchymal Stem Cells is Not Superior to G-CSF Alone." in: Frontiers in aging neuroscience, Vol. 6, pp. 130, (2014) (PubMed).
: "Rb suppresses collective invasion, circulation and metastasis of breast cancer cells in CD44-dependent manner." in: PLoS ONE, Vol. 8, Issue 12, pp. e80590, (2014) (PubMed).
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: "Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation." in: Nature communications, Vol. 10, Issue 1, pp. 5462, (2019) (PubMed).
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- Nuclei
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背景
- This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.
抗原
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