INA 抗体
Quick Overview for INA 抗体 (ABIN361343)
抗原
See all INA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for the ~66k alpha Internexin protein. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- most mammals
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纯化方法
- Protein G purified culture supernatant
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免疫原
- Recombinant rat alpha-internexin expressed in and purified from E. coli
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亚型
- IgG1
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anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) (Internal Region) antibody
INA 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) (AA 251-350) antibodyINA 适用: 人, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) antibodyINA 适用: 大鼠 WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) (AA 71-161) antibodyINA 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) (AA 290-319) antibodyINA 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB13854 unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) (AA 290-319) antibodyINA 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 257CT7-1-2 unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) antibodyINA 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 7T2 unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) antibodyINA 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) antibodyINA 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Internexin Neuronal Intermediate Filament Protein, alpha (INA) antibodyINA 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Recommended Dilution: WB: 1:2,000 IF: 1:250 IHC: 1:1,000 Quality Control: Western blots performed on each lot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- total IgG fraction + 10 mM Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))
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别名
- INA
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背景
- Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5). Anti-alpha Internexin Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein.
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分子量
- '66 kDa
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基因ID
- 24503
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UniProt
- P23565
抗原
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