NEK2 抗体 (Middle Region)
Quick Overview for NEK2 抗体 (Middle Region) (ABIN360538)
抗原
See all NEK2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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特异性
- This antibody reacts to NEK2.
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human NEK2.
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亚型
- Ig Fraction
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anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 148-397) antibody
NEK2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 1-445) antibodyNEK2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 1-445) antibodyNEK2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 282-295) antibodyNEK2 适用: 人 WB 宿主: 绵羊 Monoclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C8 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (C-Term) antibodyNEK2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 125-393) antibodyNEK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 396-426) antibodyNEK2 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
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别名
- NEK2
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背景
- Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: HSPK 21, NLK1, NimA-related protein kinase 2, Serine/threonine-protein kinase Nek2
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基因ID
- 4751
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NCBI登录号
- NP_001191111
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UniProt
- P51955
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途径
- M Phase
抗原
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