NEK1 抗体 (C-Term)
This anti-NEK1 antibody is a 兔 多克隆 antibody detecting NEK1 in WB, IHC (p) 和 EIA. Suitable for 人 和 Hamster.
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产品编号 ABIN360536
发货至:
中国
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Quick Overview for NEK1 抗体 (C-Term) (ABIN360536)
抗原
See all NEK1 抗体
NEK1
(NIMA-Related Kinase 1 (NEK1))
适用
人, Hamster
宿主
兔
克隆类型
多克隆
标记
This NEK1 antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
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抗原表位
- AA 1173-1202, C-Term
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特异性
- This antibody is specific to NEK1.
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of Human NEK1.
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亚型
- Ig Fraction
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anti-NIMA-Related Kinase 1 (NEK1) (AA 626-809) antibody
NEK1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NIMA-Related Kinase 1 (NEK1) (AA 1165-1196), (C-Term) antibodyNEK1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2957 unconjugated
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应用备注
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ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- NEK1 (NIMA-Related Kinase 1 (NEK1))
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别名
- NEK1
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背景
- Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: NimA-related protein kinase 1, NimA-related protein kinase 10, Renal carcinoma antigen NY-REN-55, Serine/threonine-protein kinase Nek1
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基因ID
- 4750
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NCBI登录号
- NP_001186326
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UniProt
- Q96PY6
抗原
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