ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
1. Mikkers, H. , et al. , Mol. Cell. Biol. 24(13): 6104-6115 (2004). 2. Eichmann, A. , et al. , Oncogene 19(9): 1215-1224 (2000).
限制
仅限研究用
状态
Liquid
浓度
0.25 mg/mL
缓冲液
PBS with 0.09 % (W/V) sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
PIM3 is a memberof the Ser/Thr protein kinase family that may be involved in cell cycle progression and anti-apoptosis process. This protein has been implicated in proliferation of human hepatoma cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine. PIM3 is expressed in human hepatoma cell lines but not in normal liver tissues.Synonyms: PIM-3, Serine/threonine-protein kinase pim-3