BCKDK 抗体 (Middle Region)
Quick Overview for BCKDK 抗体 (Middle Region) (ABIN359244)
抗原
See all BCKDK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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特异性
- This antibody reacts to BCKDK.
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纯化方法
- Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human BCKDK.
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亚型
- Ig Fraction
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anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (AA 44-223) antibody
BCKDK 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (N-Term) antibodyBCKDK 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (AA 153-412) antibodyBCKDK 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (AA 1-412) antibodyBCKDK 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) antibodyKO Validated BCKDK 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (N-Term) antibodyBCKDK 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (N-Term) antibodyBCKDK 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (AA 153-412) antibodyBCKDK 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (N-Term) antibodyBCKDK 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (AA 31-120) antibodyBCKDK 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))
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别名
- BCKDK
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背景
- The second major step in the catabolism of the branched-chain amino acids, isoleucine, leucine, and valine, is irreversibly catalyzed by the branched-chain alpha-keto acid dehydrogenase complex (BCKD), an inner-mitochondrial enzyme complex composed of 3 catalytic components: a branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). The complex also contains 2 enzymes that regulated the state of activity of the BCKD complex: a kinase (BCKDK), and a phosphorylase. The ubiquitiously expressed kinase contains 1 histidine kinase domain. Maple syrup urine disease (MSUD) is a pathology secondary to an enzyme defect in the catabolic pathway of leucine, isoleucine, and valine. Accumulation of these amino acids and their corresponding keto acids results in encephalopathy and progressive neurodegeneration in infants not treated for MSUD.Synonyms: BCKD-kinase, BCKDHKIN, Branched-chain alpha-ketoacid dehydrogenase kinase, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial
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基因ID
- 10295, 9606
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UniProt
- O14874
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途径
- SARS-CoV-2 Protein Interactome
抗原
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