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NCOA1 抗体 (pTyr215)

This anti-NCOA1 antibody is a 兔 多克隆 antibody detecting NCOA1 in WB 和 EIA. Suitable for 人.
产品编号 ABIN358229
发货至: 中国

Quick Overview for NCOA1 抗体 (pTyr215) (ABIN358229)

抗原

See all NCOA1 抗体
NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

适用

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宿主

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克隆类型

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多克隆

标记

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This NCOA1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 抗原表位

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    pTyr215

    特异性

    This antibody detects Src pTyr215. Predicted to cross react with Mouse (100 % Antigen Homology).

    纯化方法

    Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y215 of human Src.

    亚型

    Ig Fraction
  • 应用备注

    ELISA: 1/1,000. Western Blot: 1/100-1/500.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

    别名

    SRC1

    背景

    This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

    分子量

    59704 Da

    基因ID

    6714, 9606

    UniProt

    P12931

    途径

    Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
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