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p107 抗体 (pSer975)

This anti-p107 antibody (ABIN358209) is a Rabbit Polyclonal antibody detecting p107 in WB, IHC (p), EIA. Suitable for Human.
产品编号 ABIN358209
发货至: 中国

Quick Overview for p107 抗体 (pSer975) (ABIN358209)

抗原

See all p107 (RBL1) 抗体
p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

适用

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宿主

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克隆类型

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多克隆

标记

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This p107 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
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    pSer975

    特异性

    This antibody detects Rb-like-1 pSer975.

    纯化方法

    Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like 1.

    亚型

    Ig Fraction
  • 应用备注

    ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50approx. 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

    别名

    RBL1

    背景

    RB-like 1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and RB-like 1 can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that RB-like 1 may also be a tumor suppressor.Synonyms: 107 kDa retinoblastoma-associated protein, Retinoblastoma-like protein 1, p107, pRb1

    分子量

    120877 Da

    基因ID

    5933, 9606

    UniProt

    P28749

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases
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