PKMYT1 抗体 (pThr495)
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Quick Overview for PKMYT1 抗体 (pThr495) (ABIN358168)
抗原
See all PKMYT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr495
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特异性
- This antibody detects MYT1 pThr495.
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纯化方法
- Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T495 of human MYT1.
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亚型
- Ig Fraction
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anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 1-499) antibody
PKMYT1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 400-499) antibodyPKMYT1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2A3 unconjugated
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 900-1101) antibodyPKMYT1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 49-98) antibodyPKMYT1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) antibodyPKMYT1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB1530 unconjugated Recombinant Antibody
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (pSer83) antibodyPKMYT1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 1-499) antibodyPKMYT1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 452-482), (C-Term) antibodyPKMYT1 适用: 人 WB 宿主: 兔 Polyclonal RB09867 unconjugated
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 114-143), (N-Term) antibodyPKMYT1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB34667 unconjugated
anti-Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (Internal Region) antibodyPKMYT1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))
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别名
- PKMYT1
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背景
- The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: MYT1, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase
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分子量
- 54521 Da
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基因ID
- 9088, 9606
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UniProt
- Q99640
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途径
- Mitotic G1-G1/S Phases, M Phase
抗原
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