Histone H3.1 抗体 (pSer10)
Our Local Distributor
北京 101111
Quick Overview for Histone H3.1 抗体 (pSer10) (ABIN358137)
抗原
See all Histone H3.1 (HIST1H3B) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pSer10
-
特异性
- This antibody detects HIST1H3B3 pSer10.
-
纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
-
免疫原
- This antibody is generated from rabbits immunized with MAPS conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B.
-
亚型
- Ig Fraction
-
-
-
-
应用备注
-
ELISA: 1/1,000. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.25 mg/mL
-
缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
-
- Histone H3.1 (HIST1H3B)
-
别名
- Histone H3.1
-
背景
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.Synonyms: H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, H3FA, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
-
分子量
- 15273 Da
-
基因ID
- 8350, 9606
-
UniProt
- P68431
抗原
-