Histone H3.1 抗体 (pSer10)
Quick Overview for Histone H3.1 抗体 (pSer10) (ABIN358137)
抗原
See all Histone H3.1 (HIST1H3B) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer10
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特异性
- This antibody detects HIST1H3B3 pSer10.
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纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with MAPS conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B.
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亚型
- Ig Fraction
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anti-Histone H3.1 (HIST1H3B) (pSer10) antibody
HIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (pSer28) antibodyHIST1H3B 适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 5D10D4 unconjugated
anti-Histone H3.1 (HIST1H3B) (AA 21-39) antibodyHIST1H3B 适用: 人 WB, IF, ChIP, ICC, ChIP-seq 宿主: 小鼠 Monoclonal 1D4F2 unconjugated
anti-Histone H3.1 (HIST1H3B) (pSer28), (Ser28) antibodyHIST1H3B 适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 5D10D4 unconjugated
anti-Histone H3.1 (HIST1H3B) (AA 1-50), (pSer10) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (Ser10) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (pSer28) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Histone H3.1 (HIST1H3B)
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别名
- Histone H3.1
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背景
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.Synonyms: H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, H3FA, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
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分子量
- 15273 Da
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基因ID
- 8350, 9606
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UniProt
- P68431
抗原
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