UBE2E3 抗体 (N-Term)
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Quick Overview for UBE2E3 抗体 (N-Term) (ABIN357598)
抗原
See all UBE2E3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody is specific to UBCH9/UBE2E3 (N-term).
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBCH9.
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亚型
- Ig Fraction
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))
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别名
- UBE2E3
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背景
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH9 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100 % sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found.Synonyms: UBCE4, UBCH9, UbcM2, Ubiquitin carrier protein E3, Ubiquitin-conjugating enzyme E2 E3, Ubiquitin-conjugating enzyme E2-23 kDa, Ubiquitin-protein ligase E3
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分子量
- 22912 Da
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基因ID
- 10477, 9606
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UniProt
- Q969T4
抗原
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