UBE2V1 抗体 (C-Term, Isoform 3)
Quick Overview for UBE2V1 抗体 (C-Term, Isoform 3) (ABIN357577)
抗原
See all UBE2V1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term, Isoform 3
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特异性
- This antibody is specific to UBE2V1 (C-term, Isoform 3). Predicted to cross react with Mouse and Zebrafish (100 % Antigen Homology).
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2V1.
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亚型
- Ig Fraction
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anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 1-147) antibody
UBE2V1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) antibodyUBE2V1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 1-147) antibodyUBE2V1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 1-103) antibodyUBE2V1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 113-145) antibodyUBE2V1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 2-147) antibodyUBE2V1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (C-Term) antibodyUBE2V1 适用: 人, 小鼠, 大鼠, Cow, 犬, 山羊, 马, Pig, 兔, Bat, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 113-145), (C-Term) antibodyUBE2V1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB4387 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) antibodyUBE2V1 适用: 人, 小鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: 1/1,000. Western Blot: 1/100-1/500.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- UBE2V1 (Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1))
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别名
- UBE2V1 / CROC1
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背景
- The CROC1 isoforms, also known as UBE2V1, show sequence similarity to ubiquitin-conjugating enzymes (UBCs, or E2s) but lack the conserved cysteine residue critical to catalytic activity of E2s.1 Northern blot analysis detected approximately 2.1- and 2.5-kb CROC1 transcripts in all human tissues examined, with the highest levels in brain, skeletal muscle, and kidney. Partial human intestinal epithelial cell cDNAs have been isolated containing the 3-primecoding sequence and 3-prime untranslated region of UBE2V1, also called UEV1.2 UEV1 does not have ubiquitin-conjugating activity in vitro. UEV1 transcripts are downregulated upon differentiation of a colon carcinoma cell line.1 Constitutive expression of exogenous UEV1 protein in these colon carcinoma cells inhibits their capacity to differentiate upon confluence and induces changes in cell cycle behavior associated with inhibition of CDK1. A heterodimeric protein complex has been identified that links TRAF6 to IKK activation.3 Peptide mass fingerprinting analysis revealed that this complex is composed of the ubiquitin conjugating enzyme UBC13 and the UBC-like protein UBE2V1, also called UEV1A. TRAF6, a RING domain protein, functions together with UBC13/UEV1A to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 (K63) of ubiquitin. Blockade of this polyubiquitin chain synthesis, but not inhibition of the proteasome, prevents the activation of IKK by TRAF6. These results unveil a new regulatory function for ubiquitin, in which IKK is activated through the assembly of K63-linked polyubiquitin chains.Synonyms: CROC-1, P/OKcl.19, TRAF6-regulated IKK activator 1 beta Uev1A, UBE2V, UEV-1, UEV1, Ubiquitin-conjugating enzyme E2 variant 1
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分子量
- 19307 Da
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基因ID
- 387522
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NCBI登录号
- NP_001027459
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UniProt
- Q13404
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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