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- 抗原 See all USP25 抗体
- USP25 (Ubiquitin Specific Peptidase 25 (USP25))
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抗原表位
- C-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This USP25 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is specific to USP25 (C-term).
- 纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- 免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human USP25.
- 亚型
- Ig Fraction
- Top Product
- Discover our top product USP25 Primary Antibody
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anti-Ubiquitin Specific Peptidase 25 (USP25) (C-Term) antibody
USP25 适用: 人, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) antibodyUSP25 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) (AA 348-606), (C-Term) antibodyUSP25 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) (N-Term) antibodyUSP25 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) antibodyUSP25 适用: 人 WB 宿主: 小鼠 Monoclonal 7C1 unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) (AA 1-230) antibodyUSP25 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) (AA 1037-1068), (C-Term) antibodyUSP25 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB4365 unconjugated
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- 应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- USP25 (Ubiquitin Specific Peptidase 25 (USP25))
- 别名
- USP25 (USP25 产品)
- 别名
- USP25 antibody, zgc:55791 antibody, wu:fi33c05 antibody, wu:fo67e06 antibody, wu:fp97a04 antibody, usp25 antibody, MGC81666 antibody, usp21 antibody, USP21 antibody, ubiquitin specific peptidase 25 antibody, universal stress protein UspA antibody, ubiquitin specific peptidase 25 L homeolog antibody, USP25 antibody, RR_RS03930 antibody, usp25 antibody, usp25.L antibody, Usp25 antibody
- 背景
- Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.Synonyms: Deubiquitinating enzyme 25, USP on chromosome 21, USP21, Ubiquitin carboxyl-terminal hydrolase 25, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25
- 分子量
- 125749 Da
- 基因ID
- 29761, 5874
- UniProt
- Q9UHP3
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