UCHL5 抗体 (N-Term)
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Quick Overview for UCHL5 抗体 (N-Term) (ABIN357513)
抗原
See all UCHL5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody is specific to UCHL5 (N-term).
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UCHL5.
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亚型
- Ig Fraction
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anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) antibody
UCHL5 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) antibodyKD Validated UCHL5 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB4580 unconjugated Recombinant Antibody
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) (AA 1-328) antibodyUCHL5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) (AA 1-326) antibodyUCHL5 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) (AA 2-316) antibodyUCHL5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) (C-Term) antibodyUCHL5 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 斑马鱼, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) (AA 56-87) antibodyUCHL5 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) antibodyUCHL5 适用: 人 WB 宿主: 小鼠 Monoclonal 8G6 unconjugated
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) (AA 179-228) antibodyUCHL5 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 斑马鱼, 豚鼠, Pig, 小鸡, Hamster, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) antibodyUCHL5 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))
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别名
- UCHL5
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背景
- Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.Synonyms: AD-019, CGI-70, UCH-L5, UCH37, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin thioesterase L5
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分子量
- 37641 Da
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基因ID
- 51377, 9606
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UniProt
- Q9Y5K5
抗原
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