Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
免疫原
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE4A.
亚型
Ig Fraction
应用备注
ELISA 1: 1,000. Western blot 1: 100 - 1: 200. Immunohistochemistry 1: 50 - 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
0.25 mg/mL
缓冲液
PBS with 0.09 % (W/V) sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
抗原
UBE4A
(Ubiquitination Factor E4A (UBE4A))
别名
UBE4A
背景
Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze ubiquitin chain assembly in conjunction with the first three proteins.1 Human protein KIAA0126, with a predicted 1,073 amino acid residues, is a member of the novel protein family defined by ubiquitination conjugation factor E4.2 In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eucaryotes use E4-dependent proteolysis pathways for multiple cellular functions.Synonyms: KIAA0126, Ubiquitin conjugation factor E4 A