SMURF2 抗体 (N-Term)
Quick Overview for SMURF2 抗体 (N-Term) (ABIN357471)
抗原
See all SMURF2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody detects SMURF2 at N-term.
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SMURF2.
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亚型
- Ig Fraction
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anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 601-700) antibody
SMURF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) antibodySMURF2 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 702-731), (C-Term) antibodySMURF2 适用: 人, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4107-4108 unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 1-748) antibodySMURF2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (Internal Region) antibodySMURF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) antibodySMURF2 适用: 人 WB 宿主: 小鼠 Monoclonal 1826CT865-51-28 unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (C-Term) antibodySMURF2 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))
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别名
- SMURF2
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背景
- SMURF2, a 748-amino acid ubiquitin E3 ligase that is 83 % identical to SMURF1, codes for a C2-WW-HECT domain ubiquitin ligase that associates constitutively with SMAD7. Binding to SMAD7 induces export of SMURF2 and recruitment to the activated transforming growth factor-beta receptor (TGFBR), where it causes receptor and SMAD7 degradation. A strong interaction of second and third SMURF2 WW domains has been identified with SMAD1, SMAD2, and SMAD3, but not SMAD4. Western blot analysis showed that SMURF2 selectively downregulates the transcription of SMAD2 and SMAD1, but not SMAD3. The nuclear SMURF2/phosphorylated SMAD2 interaction is requires TGFB1.Synonyms: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF 2, SMURF-2
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分子量
- 86195 Da
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基因ID
- 64750
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NCBI登录号
- NP_073576
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UniProt
- Q9HAU4
抗原
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