PDHX 抗体 (C-Term)
Quick Overview for PDHX 抗体 (C-Term) (ABIN357414)
抗原
See all PDHX 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody detects PDX1 at C-term.
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from selected from the C-terminal region of human PDX1.
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亚型
- Ig Fraction
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anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (AA 220-468) antibody
PDHX 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (AA 222-471) antibodyPDHX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (AA 1-300) antibodyPDHX 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (AA 1-501) antibodyPDHX 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (AA 1-501) antibodyPDHX 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (AA 1-109) antibodyPDHX 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (C-Term) antibodyVerified PDHX 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (AA 1-300) antibodyPDHX 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (AA 54-501) antibodyPDHX 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal AT1E11 unconjugated
anti-Pyruvate Dehydrogenase Complex, Component X (PDHX) (C-Term) antibodyPDHX 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- PDHX (Pyruvate Dehydrogenase Complex, Component X (PDHX))
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别名
- PDHX / PDX1
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背景
- PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.Synonyms: Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein, E3BP, Lipoyl-containing pyruvate dehydrogenase complex component X, Pyruvate dehydrogenase protein X component mitochondrial, proX
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分子量
- 54122 Da
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基因ID
- 8050, 9606
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UniProt
- O00330
抗原
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