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GFAP 抗体 (N-Term)

There are 4+ publications for this product available. The 兔 多克隆 anti-GFAP antibody is suitable to detect GFAP in samples from 人. It has been validated for WB, IHC (p) 和 EIA.
产品编号 ABIN357395
发货至: 中国
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Quick Overview for GFAP 抗体 (N-Term) (ABIN357395)

抗原

See all GFAP 抗体
GFAP (Glial Fibrillary Acidic Protein (GFAP))

适用

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多克隆

标记

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This GFAP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
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    N-Term

    特异性

    This antibody detects GFAP N-term.

    纯化方法

    Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GFAP.

    亚型

    Ig Fraction
  • 应用备注

    ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS with 0.09 % (W/V) sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • Curtis, Martin, Gabel, Sidhu, Rzesiewicz, Mandeville, Van Gorp, Leerink, Tadokoro, Marsala, Jamieson, Marsala, Ciacci: "A First-in-Human, Phase I Study of Neural Stem Cell Transplantation for Chronic Spinal Cord Injury." in: Cell stem cell, Vol. 22, Issue 6, pp. 941-950.e6, (2018) (PubMed).

    Kadoya, Lu, Nguyen, Lee-Kubli, Kumamaru, Yao, Knackert, Poplawski, Dulin, Strobl, Takashima, Biane, Conner, Zhang, Tuszynski: "Spinal cord reconstitution with homologous neural grafts enables robust corticospinal regeneration." in: Nature medicine, Vol. 22, Issue 5, pp. 479-87, (2017) (PubMed).

    Lu, Ceto, Wang, Graham, Wu, Kumamaru, Staufenberg, Tuszynski: "Prolonged human neural stem cell maturation supports recovery in injured rodent CNS." in: The Journal of clinical investigation, Vol. 127, Issue 9, pp. 3287-3299, (2017) (PubMed).

    Miyanohara, Kamizato, Juhas, Juhasova, Navarro, Marsala, Lukacova, Hruska-Plochan, Curtis, Gabel, Ciacci, Ahrens, Kaspar, Cleveland, Marsala: "Potent spinal parenchymal AAV9-mediated gene delivery by subpial injection in adult rats and pigs." in: Molecular therapy. Methods & clinical development, Vol. 3, pp. 16046, (2016) (PubMed).

  • 抗原

    GFAP (Glial Fibrillary Acidic Protein (GFAP))

    别名

    GFAP

    背景

    GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.Synonyms: Glial Fibrillary Acidic Protein

    分子量

    49880 Da

    基因ID

    2670

    NCBI登录号

    NP_001124491

    UniProt

    P14136
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