DKK3 抗体 (N-Term)
Quick Overview for DKK3 抗体 (N-Term) (ABIN357071)
抗原
See all DKK3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody detects DKK3 at N-term.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DKK3.
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亚型
- Ig Fraction
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anti-DKK3 (AA 23-349) antibody
适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-DKK3 (AA 91-350) antibody适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 8A5C6 unconjugated
anti-DKK3 (AA 91-350) antibody适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-DKK3 (AA 31-130) antibody适用: 小鼠, 兔 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DKK3 (AA 22-350) antibody适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DKK3 (AA 22-350) antibody适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DKK3 antibody适用: 人, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4G7 unconjugated
anti-DKK3 (AA 41-348) antibody适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-DKK3 (C-Term) antibodyVerified 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-DKK3 antibody适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- DKK3
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别名
- Dickkopf-3 (DKK3)
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背景
- DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC
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分子量
- 38291 Da
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基因ID
- 27122
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NCBI登录号
- NP_001018067
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UniProt
- Q9UBP4
抗原
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