ERAS 抗体 (N-Term)
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北京 101111
Quick Overview for ERAS 抗体 (N-Term) (ABIN356980)
抗原
See all ERAS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody detects ERAS at N-term.
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纯化方法
- Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ERAS.
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亚型
- Ig Fraction
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anti-ES cell expressed Ras (ERAS) antibody
ERAS 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (C-Term) antibodyERAS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 19-52), (N-Term) antibodyERAS 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB51538 unconjugated
anti-ES cell expressed Ras (ERAS) (AA 202-236), (C-Term) antibodyERAS 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB52380 unconjugated
anti-ES cell expressed Ras (ERAS) (AA 13-42), (N-Term) antibodyERAS 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 3-230) antibodyERAS 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (C-Term) antibodyERAS 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 1-233) antibodyERAS 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) antibody (Biotin)ERAS 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
anti-ES cell expressed Ras (ERAS) antibody (FITC)ERAS 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
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应用备注
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ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunofluorescence 1: 10 - 1: 50. Immunohistochemistry 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- ERAS (ES cell expressed Ras (ERAS))
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别名
- ERAS / HRAS2
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背景
- Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.Synonyms: E-Ras, Embryonic stem cell-expressed Ras, GTPase ERas, HRASP
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分子量
- 25156 Da
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基因ID
- 3266, 9606
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UniProt
- Q7Z444
抗原
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