GST 抗体
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北京 101111
Quick Overview for GST 抗体 (ABIN356807)
抗原
See all GST 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a recombinant full length of GST.
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亚型
- Ig Fraction
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应用备注
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ELISA: 1/1,000. Western blot: 1/100-1/500Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- GST (Glutathione S Transferase (GST))
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别名
- Glutathione-S-Transferase (GST)
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背景
- Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.Synonyms: GST26-Tag, Glutathione S-transferase Tag
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基因ID
- 6182
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UniProt
- P08515
抗原
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