Ubiquitin 抗体 (C-Term)
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Quick Overview for Ubiquitin 抗体 (C-Term) (ABIN356747)
抗原
See all Ubiquitin (Ub) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 44-73, C-Term
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特异性
- This antibody is specific to Ubiquitin (C-term).
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- KLH conjugated synthetic peptide between 44~73 amino acids from the C-terminal region of Human Ubiquitin. Genename: UBC
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亚型
- Ig Fraction
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应用备注
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ELISA: 1/1,000. Western blot: 1/100-1/500. Immunofluorescence. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS, 0.09 % (W/V) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Ubiquitin (Ub)
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别名
- Ubiquitin
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背景
- This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.Synonyms: Polyubiquitin-C, UBC
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分子量
- 8.565 kDa
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基因ID
- 7316
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NCBI登录号
- NP_066289
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UniProt
- P0CG48
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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