UBE2I 抗体 (N-Term)
Quick Overview for UBE2I 抗体 (N-Term) (ABIN356594)
抗原
See all UBE2I 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody is specific to UBE2I (N-term). Predicted to cross react with Mouse and DANRE (100 % Antigen Homology).
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2I.
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亚型
- Ig Fraction
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anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) antibody
UBE2I 适用: 人, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-158) antibodyUBE2I 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibodyVerified UBE2I 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibodyUBE2I 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-30) antibodyUBE2I 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) antibodyUBE2I 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-30), (N-Term) antibodyUBE2I 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2811 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 33-158) antibodyUBE2I 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-157) antibodyUBE2I 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) antibodyUBE2I 适用: 人, 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal 7A9 unconjugated
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应用备注
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ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
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别名
- UBC9 / UBE2I
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背景
- UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBalpha, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I
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基因ID
- 7329, 9606
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UniProt
- P63279
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
抗原
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