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UBE2I 抗体 (N-Term)

This anti-UBE2I antibody is a 兔 多克隆 antibody detecting UBE2I in WB, IHC (p) 和 EIA. Suitable for 人.
产品编号 ABIN356594
发货至: 中国
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Quick Overview for UBE2I 抗体 (N-Term) (ABIN356594)

抗原

See all UBE2I 抗体
UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

适用

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宿主

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克隆类型

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多克隆

标记

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This UBE2I antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 抗原表位

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    N-Term

    特异性

    This antibody is specific to UBE2I (N-term). Predicted to cross react with Mouse and DANRE (100 % Antigen Homology).

    纯化方法

    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2I.

    亚型

    Ig Fraction
  • 应用备注

    ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS containing 0.09 % (W/V) Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

    别名

    UBC9 / UBE2I

    背景

    UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBalpha, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I

    基因ID

    7329, 9606

    UniProt

    P63279

    途径

    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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