UBE2I 抗体 (N-Term)
Our Local Distributor
北京 101111
Quick Overview for UBE2I 抗体 (N-Term) (ABIN356594)
抗原
See all UBE2I 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
特异性
- This antibody is specific to UBE2I (N-term). Predicted to cross react with Mouse and DANRE (100 % Antigen Homology).
-
纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
-
免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2I.
-
亚型
- Ig Fraction
-
-
-
-
应用备注
-
ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.25 mg/mL
-
缓冲液
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
-
- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
-
别名
- UBC9 / UBE2I
-
背景
- UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBalpha, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I
-
基因ID
- 7329, 9606
-
UniProt
- P63279
-
途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
抗原
-