MBD3 抗体 (C-Term)
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Quick Overview for MBD3 抗体 (C-Term) (ABIN356576)
抗原
See all MBD3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody is specific to MBD3 (C-term).
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纯化方法
- Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding E279 of human MBD3.
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亚型
- Ig Fraction
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anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 79-209) antibody
MBD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB695 unconjugated Recombinant Antibody
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB10 unconjugated Recombinant Antibody
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-255) antibodyMBD3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 70-179) antibodyMBD3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibodyMBD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-291) antibodyMBD3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB 宿主: 小鼠 Monoclonal 5C3 unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibodyMBD3 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
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别名
- MBD3
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背景
- DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).Synonyms: Methyl-CpG-binding domain protein 3
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基因ID
- 53615, 9606
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UniProt
- O95983
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途径
- Chromatin Binding
抗原
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