Fibronectin 抗体
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Quick Overview for Fibronectin 抗体 (ABIN356451)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Human Fibronectin in liver, tonsil, skin and kidney. Cellular localization: Connective tissue matrix and basement membrane.
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Affinity chromatography on Protein A
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免疫原
- Purified human Fibronectin from human plasma.
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亚型
- IgG
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应用备注
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Immunohistochemistry: 1/100-1/400 for 30 minute at room temp. using polymer system orstreptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections andcell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temp. for 20 minutes. Positive control: Human kidney.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 2.0 mg/mL
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缓冲液
- 10 mM PO4, 150 mM NaCl, pH 7.4, 0.05 % Sodium Azide, 1 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Fibronectin
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背景
- Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.Synonyms: CIG, Cold-insoluble globulin, FN1
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基因ID
- 2335
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NCBI登录号
- NP_002017
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UniProt
- P02751
抗原
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