UBE2I 抗体
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北京 101111
Quick Overview for UBE2I 抗体 (ABIN356383)
抗原
See all UBE2I 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody is specific to UBC9.
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产品特性
- Synonyms: UBCE9, Ube2i, Ubce2i, SUMO-protein ligase, SUMO-conjugating enzyme UBC9,Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I,Ubiquitin carrier protein 9
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纯化方法
- Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- purified GST-UBC9 fusion protein.
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亚型
- IgG1
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应用备注
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ELISA: 1/1,000. Western blotting: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
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别名
- UBC9 / UBE2I
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背景
- Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IκBalpha, and PML without the requirement of an E3 ligase. Ubc9 is essential for nuclear architecture and chromosome segregation.Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I
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基因ID
- 7329
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UniProt
- P63279
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
抗原
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