GFAP 抗体
Quick Overview for GFAP 抗体 (ABIN351523)
抗原
See all GFAP 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Species cross-reactivity: human, rat and chicken.
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纯化方法
- Protein G purified
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免疫原
- Porcine spinal cord. Cellular Localization: Cytoplasmic.
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亚型
- IgG1
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应用备注
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Approved: IF, IHC, IHC-P (1 - 2 μg/mL), IP (2 μg/tst), WB (1 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1-2 μg/mL. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells. -
说明
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Target Species of Antibody: Pig
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.2 % BSA, 0.1 % sodium azide, 40 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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别名
- GFAP
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背景
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Name/Gene ID: GFAP
Synonyms: GFAP -
基因ID
- 2670
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UniProt
- P14136
抗原
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