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GFAP 抗体

This anti-GFAP antibody is a 小鼠 单克隆 antibody detecting GFAP in WB, IHC, IF, IHC (p) 和 IP. Suitable for 人, 大鼠, Pig 和 小鸡.
产品编号 ABIN351523
发货至: 中国

Quick Overview for GFAP 抗体 (ABIN351523)

抗原

See all GFAP 抗体
GFAP (Glial Fibrillary Acidic Protein (GFAP))

适用

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人, 大鼠, Pig, 小鸡

宿主

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小鼠

克隆类型

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单克隆

标记

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This GFAP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
  • 品牌

    IHC-plus™

    特异性

    Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Species cross-reactivity: human, rat and chicken.

    纯化方法

    Protein G purified

    免疫原

    Porcine spinal cord. Cellular Localization: Cytoplasmic.

    亚型

    IgG1
  • 应用备注

    Approved: IF, IHC, IHC-P (1 - 2 μg/mL), IP (2 μg/tst), WB (1 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1-2 μg/mL. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.

    说明

    Target Species of Antibody: Pig

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide, 40 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    GFAP (Glial Fibrillary Acidic Protein (GFAP))

    别名

    GFAP

    背景

    Name/Gene ID: GFAP

    Synonyms: GFAP

    基因ID

    2670

    UniProt

    P14136
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