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ADAM12 抗体 (AA 2-14)

This 山羊 多克隆 antibody specifically detects ADAM12 in ELISA, WB, IHC 和 IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN351478
发货至: 中国
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Quick Overview for ADAM12 抗体 (AA 2-14) (ABIN351478)

抗原

See all ADAM12 抗体
ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This ADAM12 antibody is un-conjugated

应用范围

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ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 2-14

    特异性

    Human ADAM12. This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.

    预测反应

    Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide AARPLPVSPARALC from the N-terminus of human ADAM12 (NP_003465.3, NP_067673.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 0.3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

    别名

    ADAM12

    背景

    Name/Gene ID: ADAM12
    Subfamily: Metallopeptidase M12B
    Family: Protease

    Synonyms: ADAM12, ADAM 12, MCMPMltna, MCMP, MLTN, Meltrin-alpha, MLTNA

    基因ID

    8038

    NCBI登录号

    NP_003465, NP_067673

    UniProt

    O43184

    途径

    EGFR Signaling Pathway
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