A synthetic peptide from the internal region of human Rho related GTP binding protein RhoU (CDC42 like GTPase 1 CDC42L1 Ryu GTPase) conjugated to an immunogenic carrier protein was used as the antigen. The antigen shares 90% identity with rat and mouse sequence.
RHOU
适用: 小鼠, 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 350
应用备注
IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Function: Acts upstream of PAK1 to regulate the actin cytoskeleton adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Subcellular location: Cell membrane, Lipid-anchor, Cytoplasmic side. Golgi apparatus membrane, Lipid-anchor. Cell junction ‰ focal adhesion. Cell projection ‰ podosome. Note: Localizes to podosomes in SRC-transformed cells. Tissue specificity: Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach small intestine brain skeletal muscle and placenta.