A synthetic peptide from the internal part of human LIMK2 (LIM domain kinase 2 LIMK-2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat mouse.
IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
FUNCTION: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. TISSUE SPECIFICITY: Highest expression in the placenta, moderate level in liver lung kidney and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver colon stomach and spleen while in brain kidney and placenta LIMK2b is the dominant form. In adult lung both LIMK2a and LIMK2b is nearly equally observed.