Cofilin 抗体
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北京 101111
Quick Overview for Cofilin 抗体 (ABIN7698698)
抗原
See all Cofilin (CFL1) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Sheep antibody to Cofilin-1
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特异性
- Specific for Cofilin-1.
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 (详细)
- Other species not yet tested.
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纯化方法
- IgG
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免疫原
- A synthetic peptide from the phospho Y140 & S156 of human Cofilin-1 (Cofilin non-muscle isoform) conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat mouse and monkey.
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亚型
- IgG
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应用备注
- IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognice the unphosphorylated form. Not tested in other applications.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
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注意事项
- Avoid freeze and thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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有效期
- 12 months
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- Cofilin (CFL1) (Cofilin 1 (CFL1))
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别名
- Cofilin-1
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背景
- FUNCTION: Controls reversibly actin polymerization and depolymerization in a pH -sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. SUBCELLULAR LOCATION: Nucleus matrix. Cytoplasm cytoskeleton. Note: Almost completely in nucleus in cells exposed to heat shock or 10 % dimethyl sulfoxide. TISSUE SPECIFICITY: Widely distributed in various tissues.
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UniProt
- P23528
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途径
- Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
抗原
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