Actin 抗体 (C-Term)
Quick Overview for Actin 抗体 (C-Term) (ABIN350118)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Rabbit antibody to Actin
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特异性
- Specific for Actin.
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 (详细)
- Other species not yet tested.
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纯化方法
- IgG
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免疫原
- A synthetic peptide from the c-terminal of beta gamma kappa Actin (ACTB ACTG ACTK) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including rat mouse zebra fish and xenopus.
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亚型
- IgG
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应用备注
- IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
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注意事项
- Avoid freeze and thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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有效期
- 12 months
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- Actin
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背景
- FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. SUBCELLULAR LOCATION: Cytoplasm cytoskeleton.
抗原
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