EpCAM 抗体
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北京 101111
Quick Overview for EpCAM 抗体 (ABIN349703)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD326 Purified
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特异性
- The mouse monoclonal antibody 323/A3 recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.
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无交叉反应
- 大鼠
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交叉反应 （详细）
- Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Human breast cancer MCF-7 cells
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亚型
- IgG1
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibodyEPCAM 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibodyEPCAM 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibodyEPCAM 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal EGP40-1373 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 FACS, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal EGP40-1384 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibodyEPCAM 适用: 人 FACS, IHC, StM 宿主: 兔 Monoclonal EGP40-1555R unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rEGP40-1372 unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal EGP40-1372 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibodyEPCAM 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7B5C10 unconjugated
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应用备注
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Western blotting: Non-reducing conditions, recommended dilution: 1-2 μg/mL, positive control: MCF-7 cells.
Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 mL lysate.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg/mL, pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37 °C, 1 mg / ml Tris-HCl, pH 2.0), positive tissue: breast carcinoma.
Immunocytochemistry: Recommended dilution: 1-10 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM)." in: Digestive diseases and sciences, Vol. 48, Issue 12, pp. 2306-9, (2004) (PubMed).
: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, (2003) (PubMed).
: "Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions." in: Molecular and cellular biology, Vol. 21, Issue 7, pp. 2570-80, (2001) (PubMed).
: "Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4833-43, (1998) (PubMed).
: "Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion." in: Cell adhesion and communication, Vol. 2, Issue 5, pp. 417-28, (1995) (PubMed).
: "Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule." in: The Journal of cell biology, Vol. 125, Issue 2, pp. 437-46, (1994) (PubMed).
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: "Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM)." in: Digestive diseases and sciences, Vol. 48, Issue 12, pp. 2306-9, (2004) (PubMed).
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- CD326
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背景
- Epithelial cell adhesion molecule,CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.,EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1
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基因ID
- 4072
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UniProt
- P16422
抗原
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