Catalase 抗体
(2 references)Quick Overview for Catalase 抗体 (ABIN349684)
抗原
See all Catalase (CAT) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognizes all three isoforms of Arabidopsis thaliana catalase.
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产品特性
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Expected / apparent Molecular Weight of the Antigene: 57 / 55kDa
In Chlamydomonas, HydA is present in low levels of 1 µg/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are published. to detect HydA1/A2 in Chlamydomonas extracts.The amount loaded per well corresponds to 2 µg of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 µg of artificially anaerobic induced cultures, where the HydA1 protein level is lower. This antibody is recognizing 1 ngof recombinant HydA protein. -
纯化方法
- serum
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免疫原
- KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana isoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At1g35090), catalase-3 (Q42547, At1g20620),
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anti-Catalase (CAT) (AA 401-523) antibody
CAT 适用: 人 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (Internal Region) antibodyVerified CAT 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Catalase (CAT) antibodyCAT 适用: 人, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Catalase (CAT) (AA 251-350) antibodyCAT 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (AA 32-157) antibodyCAT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (AA 1-527) antibodyCAT 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (AA 1-527) antibodyCAT 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Catalase (CAT) antibodyCAT 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (C-Term) antibodyCAT 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (AA 80-239) antibodyCAT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Recommended Dilution: 1 : 8000 (ELISA), 1 : 2000 with standard ECL (WB).
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说明
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To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 0.1 % sodium azide is added as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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储存条件
- -20 °C
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: "A drought-sensitive barley variety displays oxidative stress and strongly increased contents in low-molecular weight antioxidant compounds during water deficit compared to a tolerant variety." in: Journal of plant physiology, Vol. 170, Issue 7, pp. 633-45, (2013) (PubMed).
: "Expression and purification of soluble bio-active rice plant catalase-A from recombinant Escherichia coli." in: Journal of biotechnology, Vol. 157, Issue 1, pp. 12-9, (2012) (PubMed).
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: "A drought-sensitive barley variety displays oxidative stress and strongly increased contents in low-molecular weight antioxidant compounds during water deficit compared to a tolerant variety." in: Journal of plant physiology, Vol. 170, Issue 7, pp. 633-45, (2013) (PubMed).
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- Catalase (CAT)
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别名
- Cat
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背景
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AGI Code: At4g35090
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation. -
分子量
- expected: 57 kDa, apparent: 55 kDa
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UniProt
- Q96528, P25819, Q42547
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途径
- Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism
抗原
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