UBE2J2 抗体 (Internal Region)
Quick Overview for UBE2J2 抗体 (Internal Region) (ABIN349639)
抗原
See all UBE2J2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-b43
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Supplier
- Rockland
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原理
- UBE2J2 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human Ube2j2 protein.
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产品特性
- Synonyms: rabbit anti-UBE2J2 antibody, rabbit anti-E2 J2 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J2 antibody, Non-canonical ubiquitin-conjugating enzyme 2, Ube2j2, E2 ubiquitin-conjugating enzyme J2, NCUBE-2
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human Ube2j2 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) (AA 1-230) antibody
UBE2J2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) antibodyUBE2J2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) (C-Term) antibodyUBE2J2 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 豚鼠, 斑马鱼, Pig, Bat, 猴, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) antibodyUBE2J2 适用: 人, 小鼠, 大鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) antibodyUBE2J2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) (AA 1-100) antibodyUBE2J2 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) (AA 124-224) antibodyUBE2J2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) (AA 1-259) antibodyUBE2J2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) (AA 1-259) antibodyUBE2J2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2) (C-Term) antibodyUBE2J2 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 豚鼠, 斑马鱼, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29 kDa in size corresponding to Ube2j2 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.64 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))
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别名
- UBE2J2
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j2 and Ube2j1 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal trans-membrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.
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基因ID
- 118424, 251757431
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UniProt
- Q8N2K1
抗原
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